Flotation continue

<body topmargin=0 leftmargin=0 marginheight=0 marginwidth=0> <table cellpadding=0 cellspacing=0 border=0><tr> <td valign="top" colspan=2 height=90 BGCOLOR="#CCFF99" TEXT="#080000" bgproperties="fixed" background="04078671esnimw.png"> <div> <FONT SIZE="5" COLOR="#CCFF99" FACE="Arial" ><B>AVIANITARIAN Reference Guide </B></FONT><B>     |     </B><A HREF="index_avianitarian.htm" TARGET="_top" TITLE="AVIANITARIAN Reference Guide "><B>home</B></A></div> <div> <A HREF="microscope.htm" TARGET="_top" TITLE="The Microscope for Aviculture Microbilo">The Microscope  for Aviculture Microbilogy</A>   |   <A HREF="id103.htm" TARGET="_top" TITLE="Microscope Questions">Microscope Questions</A>   |   <A HREF="id111.htm" TARGET="_top" TITLE="History of Gram Stain">History of Gram Stain</A>   |   <A HREF="id112.htm" TARGET="_top" TITLE="Gram Staining">Gram Staining</A>   |   <A HREF="id151.htm" TARGET="_top" TITLE="Fecal Flotation">Fecal Flotation</A>   |   <B>Flotation continue</B></div> <div> </div> </td> </tr><tr> <td valign="top" width=200 BGCOLOR="#336699" TEXT="#080000" bgproperties="fixed" background="041c83b3.png"> <bR> </td> <td valign="top" height=390 width="100%" BGCOLOR="#CCFF99" TEXT="#080000"> <div> <I>Flotation continue</I></div> <div> Recipes for floatation solutions are as follows.</div> <div> Sugar Solution (sheather's solution)</div> <bR> <div> 1. Pour 355 ml of warm water into your beaker. Heat the water on high but </div> <div> not to the boiling point.</div> <bR> <div> 2. Slowly add 1 LB of granulated sugar to the water, stirring constantly. </div> <div> Allow the sugar to dissolve completely before removing from the heat source.</div> <bR> <div> 3. Add 6 ml of 40% formalin or liquid phenol after the solution has </div> <div> completely cooled.</div> <bR> <div> 4. Check the specific gravity of the solution with a hydrometer before using. </div> <div> (Hydrometers are available from scientific supply houses.)</div> <bR> <div> 5. If the specific gravity is below the desired range, heat the solution and </div> <div> add more sugar, if it is higher then add more water.</div> <bR> <div> Sodium nitrate solution:</div> <bR> <div> 1. Add about 315 g of sodium nitrate to 1 liter of water while stirring. </div> <div> Heating is not necessary but it hastens the dissolution process.</div> <bR> <div> 2. Check the specific gravity and adjust as needed.</div> <div> There are 2 others which are zinc sulfate solution and saturated sodium </div> <div> chloride solution. I would encourage Aviculturist to use the zinc sulfate solution or saturated sodium chloride solution. I suggest you buy the prepared fecalsol  before going to all this work. </div> <bR> <div> They are called Fecalyzers. You put the sample in the reservoir, then </div> <div> place the cylinder that has tiny holes or grooves into the sample and stir, </div> <div> this keeps the large pieces from floating to the top, where they would adhere to the </div> <div> coverslip. The eggs can still migrate through and be seen under your </div> <div> scope. Oddly you can make a brew using rootbeer that will work the same as </div> <div> Fecalasol.</div> <bR> <bR> </td> </tr></table></body>