Streaking plate and Culture Procedure
1. Remove the media to be inoculated from the refrigerator and allow to slowly reach room temperature.
2. Collect specimen with a sterile cotton tipped applicator. See appropriate references for the proper specimen collection techniques.
3. Roll swab back and forth across tone end of Quad 1 (EMB),covering approximately one third of the agar surface (see figure 1).
4. Repeat step three (3) for the CLED w/Bevis, Pseudosel and XLD Agars.
5. Use a sterile loop or swab (do not use additional specimin), streak through the previously inculated area of agar, then down throught he remaining portion of media in quad agar. (figure 2).
6. Repeat step five for the CLED w/Bevis Agar, Pseudosel Agar and XLD Agar.
7. Incubate the inoculated media as soon as possible at 35-37 C. Turn (Invert) plates during inoculation.
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